We are seeking a highly motivated and responsible full-time Research Technician in the laboratory of Dr. Bernardo Sabatini at Harvard Medical School. The Sabatini Lab studies the neural processes by which an animal picks its next action. They focus on the circuitry of the basal ganglia, an evolutionary conserved brain structure fundamental to motor action and selection, and seeks to understand how the regulation of synapses in this structure modify animal behavior. More information on the lab can be found on their website: https://sabatini.hms.harvard.edu/.
We are targeting curious, self-driven candidates who are passionate about the world of biology and have a strong eye for detail. This role is an opportunity to partner with a world class lab and learn from scientists who are fundamentally changing how we think about human behavior.
The role will work closely with a postdoctoral fellow and another technician investigating the brain circuits that underlie flexible behavior, specifically within the basal ganglia. This project is investigating the mechanisms by which the brain rewires in response to a change in whole-body metabolic state, how this rewiring leads to altered behavior, and how these processes go awry in human disease. The candidate will assist with stereotaxic surgeries, awake-behavior in freely moving mice, and histology. The best candidates will be comfortable handling live mice, work effectively in a team setting and independently, and have an organized, methodical approach to their work. We are ideally targeting candidates looking to stay within the lab at least 2-3 years before moving on.
To be considered for this role, please apply here – do not reach out to the lab directly.
- Work efficiently and cooperatively to assist a postdoc with experiments:
- Conduct experiments with mice in a variety of behavioral paradigms
- Performing molecular biology experiments including qPCRs, (single-cell) RNA-seq, and ELISAs
- Executing and imaging fluorescence in-situ hybridization and immunohistochemistry experiments on brain tissue
- Handle and train mice on decision making tasks
- Stereotaxic surgeries for precise delivery of viral vectors into the brain
- Dissection of neural tissue in mice
- Immunohistochemistry, histology, and microscopy on fixed brain tissue
- Assist in the maintenance of a complex mouse colony that involves breeding, culling, and genotyping animals and maintaining a database:
- Interact effectively with lab members and veterinary staff to ensure a high standard of care and organization
- Participate in laboratory meetings and read literature applicable to the research area
- Help with lab organization, including accurate documentation and recording/maintenance of large data files
- Assist with other support duties relating to lab management and operations as required, such as ordering supplies and maintaining stocks
- Keep detailed records
- Bachelor’s degree in Biology, Neuroscience, Psychology, Computer Science, Physics, Chemistry or related discipline
- Previous hands-on research laboratory experience required. Lab experience obtained during undergraduate studies is acceptable and will be considered.
- Prior experience with animal husbandry, PCR, and knowledge of basic genetics
- Prior experience with stereotaxic surgeries and animal behavior a plus
- Experience with data analysis and programming in MATLAB and/or Python a plus
- Self-motivated and able to perform complex tasks effectively and independently with general instruction
- Excellent oral and written communication skills
- Exemplary organizational skills and attention to detail
- Ability to follow instruction with an eagerness to learn, grow professionally, and be mentored